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How to do FPLC - an example of affinity chromatography

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Fast Protein Liquid Chromatography, short FPLC, is a commonly used for purification of biomolecules, including proteins via affinity chromatography. Due to the setup of hardware and the involvement of settings via a computer software, the method can be intimidating on the first try. However FPLC provides the opportunity for maximum control of all steps of your chromatography, even miniscule modifications are possible. Therefore, we created this video to explain a FPLC: We used the example of affinity chromatography by purifying His-tagged GFP using our in-house developed Ni-INDIGO Agarose resin. Ni-INDIGO resin can be found here: 🤍 And pre-packed into FPLC Ready Columns/Cartridges: 🤍 The recipes for all mentioned buffers can be found here: 🤍 Time stamps of the shown procedure: 0:00​ - Intro 0:11​ - Build-up of a FPLC system 1:15​ - How to set-up a FPLC - Buffers & Columns 1:58​ - How to configure the FPLC Software (Biologic Duoflow Example) 3:22​ - The chromatogram - Explained! 4:27​ - Fractioning & Collecting sample 4:50 - Endcard The individual steps in order: 1. Attach binding and elution buffer to your system 2. Unplug top of the affinity resin cartridge and plug it in. IMPORTANT: Ensure that not air gets into the system. 3. Do the same with the bottom plug of the affinity resin cartridge 4. Load your protein sample into the loop 5. Setup of the program - The amounts set in the protocol are optimized for His-tag GFP purification. But it can be used as a starting point for the setting of other His-tag protein purifications with His-tag affinity cartridges: The program consists of three different steps varying in their ratio between binding and elution Buffer. 5.1 Step A - Binding: 100% binding buffer and 0% elution buffer 5.2 Step B - Washing: 92% binding buffer and 8% elution buffer 5.3 Step C - Elution: 0% binding buffer and 100% elution buffer 5.4 Afterwards set up the volumes of the elution fractions. 6. Collect the fractions which contain your purified protein.

Meet the AKTA! (and don't be intimidated by it) - Fast Protein Liquid Chromatography (FPLC)

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30.06.2021

If you’ve seen my lab bench in the middle of a protein purification, you know I can make quite a mess, but when it comes to the protein itself, I’m a stickler for cleanliness. And my main go-to protein cleaning tool is protein chromatography, a way to separate proteins based on differences in their properties. That bench mess can wait while I bring my protein to our protein “laundromat” - a FPLC (Fast Protein Liquid Chromatography) machine called an AKTA. So what’s actually going on in an AKTA? See accompanying blog post for text and figures 🤍 For a longer version with more tips: 🤍

FPLC || Fast protein liquid chromatography || Fast performance liquid chromatography

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26.11.2022

In this video we will discuss about FPLC || Fast protein liquid chromatography || Fast performance liquid chromatography your queries: FPLC fast protein liquid chromatography fast performance liquid chromatography principle of fplc principle of fast performance liquid chromatography principle of fast protein liquid chromatography define fplc define fast protein liquid chromatography define past performances liquid chromatography #chromatography #chemistry

10 Things you Need to Know about Biochromatographuy - FPLC vs HPLC

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24.06.2016

In this video we outline 10 different things you need to know about biochromatography, and compare FPLC against HPLC.

how to qualify fplc?

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LOOT.RUN - 🤍 PROMO: FOCUS (+20%) how to qualify fplc? VK: 🤍 Steam: 🤍 Faceit (10 lvl): 🤍 Config: 🤍 Music: Alex Sevenrings - I have so much to play

07 - Size Exclusion Chromatography and AKTA Pure

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10.12.2020

In this video, we talk about size exclusion chromatography and a Fast Protein Liquid Chromatography (FPLC) machine that is called AKTA pure. 🤍 🤍

B-YOH x Maltès x Django DKR - FPLC (prod: Ihas Beats)

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07.02.2023

B-YOH x Maltès x DJANGO DKR - FPLC (prod Ihas Beats) Morceau dispo sur toutes les plateformes mixé par Akazera produit par Ihas beats - 🤍 réalisé par Carré dans l'axe -🤍 🤍 🤍 🤍 🤍

Instrumentation of Fast Protein liquid chromatography ( FPLC )

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03.12.2022

in this video we will discuss about Instrumentation of Fast Protein liquid chromatography ( FPLC ) , instrumentation of Fast performance liquid chromatography, instrumentation of FPLC your queries: instrumentation of Fast Protein liquid chromatography instrumentation of Fast performance liquid chromatography instrumentation of FPLC instrumentation of fplc how does FPLC is differ from HPLC #fplc #chromatography #education #chemistry #trending

what is AKTA/FPLC? || Biotech||Chromatography|| #interview #lifescience #biotechnology #csir #gate23

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#interview #lifesciencestudent #pharmaceutical #industry #microbiology #biotechnology #scientist #research #researchwriting #researcher #nobelprize #science #pw_motivation #amandhattarwal #csir #csirnet #csirnetbyjusexamprep #byjus #gate #gate2022 #gate2023 #result #neet2022 #neet #armyschool #lecture #panacea #biotechnologist #company #easy #style #shorts #nepal #earthquake #fplc #Akta #tothepoint #Thenerdsociety #nerds #korean #anime #upsc #upscaspirants #upscmotivation #youtuber #fast #shorts #shortnotes #shoryoutube #shortvideo #unacademy #ifasbestcsirnet #csir #gate #12th #12thclass #aakash #allenkota #physicswallah #physicswallah_ki_gang #pre-foundation #foundationcourse #armyschool #best #vlogs #style #lifesciencestudent #csirresults2021 #csirmathematics #csirnetdec2021 #alakhpandey #elonmusk #bjp #diwalispecial #diwali #bestgadgets #bestmomentsfreefire #cbseboard #interview #lifesciencestudent #pharmaceutical #industry #microbiology #biotechnology #scientist #research #researchwriting #researcher #nobelprize #science #pw_motivation #amandhattarwal #csir #csirnet #csirnetbyjusexamprep #byjus #gate #gate2022 #gate2023 #result #neet2022 #neet #armyschool #lecture #panacea #biotechnologist #company #easy #style #shorts #nepal #earthquake #fplc #Akta #tothepoint #Thenerdsociety #nerds #korean #anime #upsc #upscaspirants #upscmotivation #youtuber #fast #shorts #shortnotes #shoryoutube #shortvideo #unacademy #ifasbestcsirnet content credits to :- NCBI Research paper, Bio-rad paper, The Bumbling Biochemist . Teacher :- richasingh230297🤍gmail.com

Fast performance liquid chromatography (FPLC) used in research labs to purify recombinant proteins.

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12.03.2023

Hi, Shubhashish here. I am a PhD scholar working in the field of cancer biology. This video gives an overview of FPLC-based gel permeation chromatography for protein purification. Hopefully, you will find this video interesting. Don't forget to like, share, comment, and subscribe. Also, press the bell icon for notification. #shorts #research #researchersenigma #biochemistry #biotechnology #csirnet #science #scienceexperiment #scientist #molecularbiology #lifescience #phd #phdinindia /CONNECT/ 🤍

100% WINRATE IN FPLC

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21.02.2018

Link donatii: 🤍 Trade Link: 🤍 Business inquiries: costinjax🤍gmail.com 📺Devino membru: 🤍 ★If you wanna join ESEA: 🤍 ★Social media Facebok Page: 🤍 Instagram Page: 🤍 GRUP STEAM: 🤍 Twitter Page: 🤍 SteamID: 🤍 ★ Top Donators: Adi Forever grateful: HOTSTEPPER! Thanks for the support ★ About 23 Y/O, Student UPB (FIMM) CS:GO player at Nexus ★Nexus Nexus Facebook: 🤍 Nexus Shop: 🤍 Nexus Website: 🤍 ★Stream Setup: Microphone: NT Rode USB 2.0 Webcam: Logitech C920 Lights: Vio Computers XD ★My PC: Procesor Intel Coffee Lake, Core i7 8700K 3.70GHz box (5.00 OC) Placa video Nvidia GTX 1080 Placa de baza MSI Z370 GAMING PRO CARBON Cooler CPU Deepcool Maelstrom 240T Red Sursa Sirtec - High Power Element Smart 750W Monitor: BENQ XL2540 Pret: 🤍 ★GEAR: Mouse: ZA13 Headphones: Hyperx Cloud Alpha Keyboard: Logitech G810 Mousepad: Logitech G640 ESL One Edition ★CS:GO Settings: Config: 🤍 Rezolutie: 1280x960 streched (fullscreen) Tinta: 🤍 Sensivitate: 2.34 / 400DPI / m_rawinput 1 Setlaunch: -novid -nojoy -freq 144 -tickrate 128 Setari nvidia: 🤍 🤍 Setari video: 🤍 Setari audio: 🤍 Viewmodel: 🤍 ★Bazar: Sursa Be Quiet Pure Power 10 600w sigilata (380lei negociabil) Monitor Samsung 22" - 2243NW (250lei negociabil) 🤍 - 200 lei sigilata Pentru detalii: 🤍 © I do not own any copyright on the songs that are playing during my livestreams and i am not using them for comercial reasons.

Best of DEPRESHN // FPLC Qualifier Highlights // BALKAN 16 Y/O PRODIGY

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23.09.2019

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ЗНАКОМСТВО С ЛИГОЙ FPL-C | ROAD TO FPL #1

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06.01.2022

◆ TWITCH - 🤍 ◆ Telegram - 🤍 ◆ Official Page VK - 🤍 ◆ Instagram - 🤍 #fear #csgo #fplc

FPLC-Q Highlights (14-8)

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FPLC w Zakładzie Biologii Molekularnej Uniwersytetu Warszawskiego

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12.10.2020

Materiał filmowy przygotowany na potrzeby zajęć fakultatywnych "białka i kwasy nukleinowe" w roku akademickim 2019/2020, które z powodu pandemii COVID-19 odbyły się całkowicie on-line. FPLC = Fast Protein Liquid Chromatography

לייב CSGO - המסע ל FPLC @Xivi1337

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02.06.2023

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Lab demonstration: สาธิตการใช้เครื่อง FPLC

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02.11.2021

วิดิโอสาธิตการใช้เครื่อง FPLC ณ ห้อง ท. 233 ภาควิชาเทคโนโลยีชีวภาพ คณะวิศวกรรมศาสตร์และเทคโนโลยีอุตสาหกรรม มหาวิทยาลัยศิลปากร ผู้บรรยาย: พี่แพร นักศึกษาปริญญาโท

FPLC demonstration

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F.P.L.C (feat. Nahir)

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14.10.2021

Provided to YouTube by Believe SAS F.P.L.C (feat. Nahir) · Goulag · Nahir · Skdbnff Beats · Mohamed Amine Djelabi · ASB MUSIC Perpette ou les îles 2 ℗ ASB MUSIC / BPS Released on: 2021-10-15 Auto-generated by YouTube.

Advantages and disadvantages of Fast Protein liquid chromatography, application of fplc

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05.12.2022

in this video we will discuss about Advantages and disadvantages of Fast Protein liquid chromatography, application of fplc , application of fast protein liquid chromatography #chemistry #education #fplc #fplchannel 🤍KidsTvPortugues 🤍TheOrganicChemistryTutor 🤍chemistry 🤍analyticalclassroom9337

FPLC HIGHLIGHTS 2023

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15.03.2023

i lied there are no beta keys, but my settings are here 🤍 maybe 1 day i will win $$ from this hub o_O 🤍 🤍 🤍 🤍 🤍 hallelujah - joey purp thumbnail creds to 🤍kappie7063

FPLC demo part I

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10.02.2012

САШКА СМОТРИТ: focus how to qualify fplc?

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20.08.2021

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GE Healthcare AKTA Purifier 10 FPLC System

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25.01.2017

GE Healthcare AKTA Purifier 10 FPLC System available from The Lab World Group. For more information, please visit us at 🤍

Goulag feat. Nahir "F.P.L.C" #PlanèteRap

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POKA ставит 30+ ФРАГОВ на FPLC

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08.10.2021

Всем привет! Для тех, кто хочет поддержать канал: я добавил функцию "спонсорство" Кому интересно - милости прошу, всем буду рад) Поставил минимально возможную цену - 25 рублей. Всем спасибо за вашу активность и поддержку! Поддержать канал - 4276 5218 2688 2114 Поддержать канал - donationalerts.com/r/poka_luchee Сотрудничество - vitlosk🤍mail.ru POKA: TWITCH - 🤍 YT - 🤍 INST - 🤍 VK - 🤍

FPLC Fast Protein Liquid

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17.02.2022

LIVE - NEXUS GAMING JAXI NE JUCAM FPLC!!

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How i played fplc qualifiers at 15

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21.01.2020

In this video i played fpl-c quals and i actually did pretty well for my first time! One thing i have noticed that fplc quals is about 80% luck and 20% skill. Enjoy watching!!! My instagram - 🤍 My Twitch - 🤍 My Discord Server - 🤍

GE AKTA Prime Plus FPLC System

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07.08.2017

GE AKTA Prime Plus FPLC System available at The Lab World Group. For more information please visit us at: 🤍

FPLC Column Selection Considerations

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19.01.2022

"FPLC Column Selection Considerations." Presented by Dan Yukon, Head of North American Sales and Global Sales of SNAP Products, Astrea Bioseparations. BioProcess International Ask the Expert 2020.

🔴 KTRL HEAVENACHE l FACEIT 3500 ELO ROAD TO FPLC ! DAY 2

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04.05.2021

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Never FPL-C player

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🔴 VAMOS HEAVENACHE / FACEIT ROAD TO FPLC ! *MRBIT

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22.11.2021

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comeback vs fplc ??

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02.07.2020

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Contichrom CUBE bench-top FPLC - a chromatographic new tool for drug discovery and purification

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00:37:25
12.05.2020

The Contichrom systems are multi-purpose bench-top chromatography system for proteins, oligos and peptides. This 30 minute seminar illustrates how Contichrom systems allow exploring the world of continuous chromatography processes by offering process capabilities for capture (CaptureSMB), polishing (MCSGP) and side compound enrichment (N-Rich). The operating software ChromIQ facilitates transition from single column chromatography to continuous chromatography in a few minutes. This presentation shows case studies of process design and operation using the Contichrom system.

FPLC SUPERSTAR

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28.04.2023

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⭐iM- / Faceit/Fplc/rankg de plictiseala

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02:23:44
22.06.2020

♦Donation link : 🤍 ♦Trade link: 🤍 ♦Member: 🤍 ♦Instagram: 🤍 ♦Twitter: 🤍 ♦Facebook: 🤍 ♦Steam profile: 🤍 🤑Sign pe steam: 2€ 🤑Add pe steam: 10€ ►PC Specs : ♦Procesor : Intel ® Core™ i7-8700K ♦Placa video : ASUS GeFrce GTX 970 OC DirectCU II Black 4GB ♦Placa de baza : MSI Z370 GAMING PLUS ♦Ram : HyperX Fury Red 8GB DDR4 2400MHz CL15 1.2v ♦Cooler : CPU Thermaltake Water 3.0 Performer C ♦Carcasa : Genesis Irid 300 Red ►Gear : ♦Mouse : Logitech g305 ♦Keyboard : Logitech Pro X ♦Headsets : Logitech Pro X ♦Mousepad : Logitech G640 esl edition ♦Monitor : BENQ XL2411P ►CSGO Settings : ♦Rezolutie : 4:3 1152x864 ♦Crosshair : size 3; gap -2; kdot 0; thickness 0.8 ; style 4 ♦Sensitivitate : 2.35 400 dpi

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